Dear all,
the next MX-proposal application deadline: Sep 01, 2024 is approaching As usual, all proposals will be handled by our electronic user portal GATE, https://www.helmholtz-berlin.de/pubbin/hzbgate Hereby we would like to invite the submission of new proposals for MX-beamtime at the HZB-MX beamlines for the next beam time period 2025-1 (01/2025-06/2025). In order to apply for beamtime, please register in GATE and submit a new beam time application proposal. Please note that we now expect from each research group only ONE proposal, which can contain up to 20 individual projects. IMPORTANT: If you have a running 2024-1 or a running 2024-2 proposal, you may ask for extension. For a 2024-1 proposal, an interim report is necessary, and for a 2023-2 proposal a full report including highlights. After submitting the reports to GATE, you will be able to edit and modify your proposals by adding and deleting projects. HZB provides MX-beamtime at the three MX-beamlines BL14.1, BL14.2 and BL14.3. The three beamlines are equipped with state-of-the-art instrumentation and are highly productive with more than 4400 PDB depositions in total. Beamtime is granted based on the reviewed proposals and on reports from previous research activities. Please make sure to include them if available. Experimental setup: BL14.1: - Photon energy range: 5.5-16 keV (wavelength: 0.775-2.25 A) - Photon flux: 1.8x10¹¹ Phot/sec x 100 mA at sample position (0.04-1 sec exposure time per frame) - PILATUS3 S 6M detector with 1000 µm Si sensor thickness, 141 mm-680 mm max. distance from the sample Please note: in December 2024, the detector will be replaced with a PILATUS3X 6M detector featuring an even faster image acquisition. - Microdiffractometer (MD2) with Mini-kappa goniometer - Automatic sample changer (CATS), 9 Unipucks, i.e. 144 sample storage capacity - User defined beam shaping from 50 µm-100 µm diameter possible - Multi-core XEON-CPU server, with 10GB uplink to Pilatus 6M - Data collection control via MXCuBE2 - Common MX-software installed including XDS, iMOSFLM, CCP4, Phenix, SHELXC-D-E, etc. - Automated data processing using XDSAPP3 - Remotely controlled cryo-shutter for crystal annealing - AMPTEK-XRF detector and XFEPLOT software available BL14.2: - Photon energy range: 5.5-16 keV (wavelength: 0.775-2.25 A) - Photon flux: 1.6x10¹¹ Phot/sec x 100 mA at sample position (0.05-1 sec exposure time per frame) - PILATUS3 S 2M detector with 1000 µm Si sensor thickness, 85 mm - 800 mm distance from the sample (a special mode with 56 mm distance is also available upon request for ultra-high resolution studies) - Nanodiffractometer with fast air-bearing axis and on-axis sample microscope - User defined beam shaping from 30 µm-150 µm diameter possible - Data collection control via MXCuBE2 - ISARA2 sample changer for Unipuck support, dewar with 29 Unipuck slots for up to 464 samples - Multi-core XEON-CPU server, with fibre channel SAN up-link data processing environment - Common MX-software installed including XDS, iMOSFLM, CCP4, Phenix, SHELXC-D-E, etc. - Automated data processing using XDSAPP - AMPTEK-XRF detector and XFEPLOT software available - UV-Microsprectrophotometer offline setup available If you need atomic resolution or better, BL14.2 is the beamline of choice for you!! BL14.3: - Fixed photon energy: 13.8 keV (wavelength: 0.89 A) - Photon flux: 1.6x10exp10 Phot/sec x 100mA at sample position (3-20 sec exposure time per frame) - PILATUS 6M detector, 54 mm-450 mm distance from the sample - MD2S microdiffractometer with mini-kappa goniometer - RT data collection - Data collection at 50 K using a He cryostat - Multi-core XEON-CPU server, with fibre channel SAN up-link data processing environment - Data collection control via MXCuBE2 - Common MX software installed including EDNA, XDS, iMOSFLM, CCP4, Phenix, SHELXC-D-E, etc. - Automated data processing using XDSAPP - Remotely controlled cryo-shutter for crystal annealing - REX rapid nozzle exchanger - HC-Lab dehydration device installed (please specify HC-Lab-beamtime in your proposal if needed) - AMPTEK-XRF detector and XFEPLOT software available Other facilities: - Ultra high performance stereo microscope Leica M205A, 20-255x zoom, 8 Mpix CCD-camera - Pressure chamber for noble gas derivatization (Xe, Kr available upon request) S1-biolab facilities (separate registration required): - Protein production and purification (AEKTA) - nL 96-well crystallization plate formulation and storage at 5°C and 20°C - Biophysical characterization with real time PCR (thermofluor assay) The HZB-MX group is also providing expert assistance as well as access to a library of fragments for carrying out crystallographic fragment-screening experiments. For more information please see https://www.helmholtz-berlin.de/forschung/oe/ps/macromolecular-crystallography/ fragment-screening/index_en.html or contact mswe...@helmholtz-berlin.de. Please visit our web page www.helmholtz-berlin.de/bessy-mx in order to obtain updated information about our experimental setup and other requirements. Manfred Weiss, Uwe Mueller and the HZB-MX team ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
