Good morning Adewumi Adeyeye,I'll look at the problem but I need the raw data. Please upload the *.h5 files from this crystal to some cloud service, and send me the link.
Best wishes, Kay Am 12.04.24 um 10:40 schrieb Adewumi Adeyeye:
Good day,I am writing to seek your help with resolving the problems I have been having solving the structure of my protein. I collected my data on the Beamline I04 of the Diamond Light Source. There were at least 4 different types of crystals in the collection. Unfortunately, I couldn't get a solution. However, with the assistance of yourself and other experts, we identified that my best data belong to a contaminant protein. Afterwards, I decided to inspect all the other dataset. I noticed there is a dataset which reported 85% completeness. Upon analysis with Xtriage it suggested indexing at a lower space group. I suspect this dataset might belong to my protein of interest. The images show that it is my crystal of interest that was loaded giving this dataset. I would really love to explore this. I have downloaded the dataset and tried using XDS to index but my results have been inconsistent. Initially, the process was smooth all the way through and yielded hkl file. Subsequent attempt failed to even proceed beyond IDXREF. I don't know what I am doing wrong.Any help would be so much appreciated Thank you. Inline image Inline image Kind Regards, Adewumi Adeyeye, (PhD Candidate) Department of Biochemistry, Genetics, and Microbiology Structural Biology Group, Room 3-7, Agricultural Sciences Building, University of Pretoria c/o University & Lynnwood Roads Hatfield Pretoria 0083 Cell: +27638643131 South Africa
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