Hello Todd, I get the best solution for p22121 space group after MR with an LLG score of 640 from phaser. and the Rfree is .4748. However after MR refinement does not lower the Rfree and it appears to make the Rfree worse. The XDS software indicates that my best solution is P21 21 2. Often Phaser MR places the solution in P 21 21 2. The helicase is a superfamily 2 helicase and is only monomeric. Its a 543aa long protein with a MW of 62Kda. It should have two RecA like domains at the core but the protein I have crystallized has a previously uncharacterized n-terminal domain responsible for tight single stranded DNA binding. I have tried different space groups manually but that resulted in clashing. I will be frank I do need to work on my crystallography background as crystal lattices, space group, and self-rotaion functions are limited. Thank you so far for your help , I will try further trimming down my search model into separate domain and trying that in the meantime.
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