You are cordially invited to join …….. Dominique Bourgeois Institut de Biologie Structurale J-P. Ebel, GRENOBLE Wednesday, June 28, 2023, 13:30 EDT (New York time) STRUCTURAL PHOTOPHYSICS OF FLUORESCENT PROTEINS USED IN SUPER RESOLUTION MICROSCOPY https://bnl.zoomgov.com/meeting/register/vJIsf-2orTkoHue9RhbBuN5K0UKRrafNNTk Abstract Phototransformable fluorescent proteins (PTFPs) are widely used in many advanced fluorescence microscopy methods, notably in the super-resolution field. A plethora of PTFPs have been engineered in the last years, which display a variety of photoactivation, photoconversion, photoswitching, photoblinking and photobleaching properties that can be used at advantage in many super-resolution schemes, but that are also at the origin of significant complications and artifacts, making these fascinating genetically encoded labels still far from ideal. In this talk, I will review how (kinetic) X-ray crystallography, optical spectroscopy, multidimensional NMR and molecular dynamics simulations can be combined with single-molecule investigations to reveal phototransformation mechanisms of PTFPs at the near-atomic level. Graphical Abstract: [cid:653945e9-d41c-4f3d-bb0a-2b57aba4efed] =============================================================================== Vivian Stojanoff, PhD Education, Training, Outreach User Program p 1(631) 344 8375 e nsls.lifescien...@gmail.com w https://www.bnl.gov/ps/lifesciences/<https://www.bnl.gov/ps/lsbr/> Address: Center for Biomolecular Structure National Synchrotron Light Source II Building 745 Brookhaven National Laboratory Upton NY 11973 Supporting Grants: CBMS is supported by NIH-NIGMS #P30GM133893, and by the DOE-BER #KP1605010. Any work performed at NSLS-II is supported by DOE-BES under contract # DE-SC0012704. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
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