Dear Afsan,

Based on the information you provided, it's possible that the crashing
issue in COOT when attempting to merge the monomers could be due to several
reasons. Here are a few suggestions and potential workarounds you can try:

Either Update COOT or Import monomers as coordinates: Instead of using the
"Get monomer" option in COOT, you can try importing the monomers as
separate coordinate files. You can save the Glycerol or BCT coordinates in
a PDB or CIF file format and then import them into COOT using the "File"
menu or drag-and-drop functionality. This approach bypasses any potential
issues with the "Get monomer" option.

I hope these suggestions help you overcome the crashing issue in COOT while
adding monomers to your cryo-EM data.

Best regards,

Kirti



On Tue, Jun 27, 2023 at 4:01 PM Afshan Begum <
000001d12a434d1a-dmarc-requ...@jiscmail.ac.uk> wrote:

> Dear expert,
>
> I am currently working on building my cryo-EM data using COOT. However, I
> have encountered an issue when trying to add monomers such as Glycerol or
> BCT using their letter code from the "Get monomer" option in COOT. Whenever
> I attempt to merge them into my coordinate, COOT crashes.
>
> I would greatly appreciate any suggestions or advice on how to overcome
> this issue. I am currently using version 0.9.8.5 of COOT on a Mac operating
> system. I am unsure if this is a bug or if there is a specific workaround
> for this situation.
>
> Thank you for your assistance.
>
> Best Regards
> ===============================
>  Afshan Begum
> <http://de.mc517.mail.yahoo.com/mc/compose?to=bet...@unisgi1.desy.de>
>
>
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-- 
With best regards,
Kirti Sonkar
Molecular Pathology Group
International Center for Genetic Engineering and Biotechnology (ICGEB)
AREA Science Park ,
Padriciano 99, 34149
Trieste, Italy

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