Dear Kavya, 4 mM is quite a high concentration of TCEP, perhaps they are TCEP crystals.
You could try a Unit Cell Search of the CCDC to see if you find a match: Unit Cell Search - WebCSD (cam.ac.uk)<https://www.ccdc.cam.ac.uk/structures/WebCSD/UnitCell> Best, Thomas Thomas Flower, PhD Senior Scientist, Protein Science [cid:image001.png@01D937B5.E93AE6A0] Galapagos 102 Avenue Gaston Roussel 93230 Romainville France T: +33 7 81 26 95 70 www.glpg.com<http://www.glpg.com/> From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of mesters@biochem Sent: vendredi 3 février 2023 9:53 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Regarding the diffraction image You don't often get email from mest...@biochem.uni-luebeck.de<mailto:mest...@biochem.uni-luebeck.de>. Learn why this is important<https://aka.ms/LearnAboutSenderIdentification> A and B unit cell dimensions are hardly bigger than twice the uni cell of cubic NaCl and will probably not accommodate a 30 kDa protein. Make a SDS gel of washed & dissolved crystals to be sure these are not alt or inhibitor crystals. You can stain the crystals with IzIt... J. -- Dr. math. et dis. nat. Jeroen R. Mesters https://orcid.org/0000-0001-8532-6699<https://eur05.safelinks.protection.outlook.com/?url=https%3A%2F%2Forcid.org%2F0000-0001-8532-6699&data=05%7C01%7CThomas.flower%40GLPG.COM%7C44212ddb46284392893a08db05c40c61%7C627f3c33bccc48bba033c0a6521f7642%7C1%7C0%7C638110111905504664%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=BCI5EW%2B3Ha6ZJkPsy1sOX2ePH1Sa21Nm5iXg7%2F%2B64ys%3D&reserved=0> [cid:image002.png@01D937B5.E93AE6A0] University of Lübeck Institute of Biochemistry https://www.biochem.uni-luebeck.de<https://eur05.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.biochem.uni-luebeck.de%2F&data=05%7C01%7CThomas.flower%40GLPG.COM%7C44212ddb46284392893a08db05c40c61%7C627f3c33bccc48bba033c0a6521f7642%7C1%7C0%7C638110111905660899%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=W%2FqGhyhxj72dqaDanauqUn0AwROzS5M4hH3xqrz6vXY%3D&reserved=0> phone: +49-451-3101-3105 Ratzeburger Allee 160 23562 Lübeck Germany -- Am 03.02.2023 um 09:22 schrieb kavyashreem <kavyashr...@instem.res.in<mailto:kavyashr...@instem.res.in>>: Dear all, We crystallized a protein (30kDa) + ligand (by cocrystallization), in the condition 10%PEG3350, 50mM Zinc acetate. Protein was in the buffer 20mM HEPES, 150mM NaCl, 1uM ZnCl2, 4mM TCEP, pH 8. Crystal: Crystal: crystal under UV m <b06fc576.png> <e091c7fd.png> <8ef9453e.png> When we collected the data at an in-house facility, it looked something like this: <b903961d.png> The minimum resolution spot is around 9Ang and maximum ~2.2Ang. I have not come across a protein diffraction like this, nor of a salt. When I ran the gel for the incubated protein (protein+ligand), there was no degradation. Although, I was sure there is some problem with this image I tried processing, which could not be, But indexing showed a unit cell of 11Ang, 11Ang, 46Ang in P3. which was quite expected for two of the axes but not the third. Can anyone please shed some light on this diffraction image? How can it happen? Thank you Regards Kavya ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://eur05.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCCP4BB%26A%3D1&data=05%7C01%7CThomas.flower%40GLPG.COM%7C44212ddb46284392893a08db05c40c61%7C627f3c33bccc48bba033c0a6521f7642%7C1%7C0%7C638110111905660899%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=x6mbkUuRwfHe3zF3dDFIxgCOJU7uslR2iy%2FcVrLKWrY%3D&reserved=0> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://eur05.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCCP4BB%26A%3D1&data=05%7C01%7CThomas.flower%40GLPG.COM%7C44212ddb46284392893a08db05c40c61%7C627f3c33bccc48bba033c0a6521f7642%7C1%7C0%7C638110111905660899%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=x6mbkUuRwfHe3zF3dDFIxgCOJU7uslR2iy%2FcVrLKWrY%3D&reserved=0> This e-mail and its attachment(s) (if any) may contain confidential and/or proprietary information and is intended for its addressee(s) only. 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