Dear All,
Under the umbrella of the EMBL-ESRF Joint Structural Biology Group a
collaborative project to upgrade MASSIF-1 and exploit synergies with the
EMBL High Throughput Crystallization facility (HTX Lab) in Grenoble has
been established. An integral part was the installation of one of the
CrystalDirect automatic crystal harvesting robots into the beam line
environment, enabling new experimental modalities. Thanks to the hard
work of the EMBL Instrumentation, Synchrotron Crystallography and HTX
Lab Teams, and the ESRF Structural Biology group, the installation is
now completed.
We are now opening access to newly developed CrystalDirect plate-to-beam
experimental modalities at MASSIF-1 via the EMBL HTX Lab. We are
inviting applications for either collaborative or user access.
Opportunities for funded access are available through iNEXT Discovery.
If you are interested in any of the new experiments listed below please
contact us at h...@embl.fr or mass...@esrf.fr
• Automated Protein-to-structure pipelines with CrystalDirect
plate-to-beam cryogenic data collection. Either for full diffraction
data collection or rapid diffraction-based screening and prioritization
of initial crystallization hits.
• Automated high throughput room temperature data collection to help
identify conformational variability with potential insights into protein
dynamics.
• Automated crystal dehydration experiments to modulate crystal
parameters.
Pure samples can be shipped to the EMBL HTX Lab were crystallization
experiments will be carried out. Online experimental design and
real-time access to results is facilitated via the Crystallographic
Information Management System (CRIMS) web interface. Typical
experimental workflows include crystallization screening and
optimization, crystal harvesting and CrystalDirect-plate-to-beam data
collection at MASSIF-1.
contact h...@embl.fr or mass...@esrf.fr
Best wishes
_________________________________________________________
Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://www.embl.org/groups/marquez/
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/
https://htxlab.embl.fr/
_________________________________________________________
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