Dear Jon,
We did exactly the same back in 1999's.
1) Natesh R, Bhanumoorthy P, Vithayathil PJ, Sekar K, Ramakumar S,
Viswamitra MA. (1999). Crystal structure at 1.8 Å resolution and proposed
amino acid sequence of a thermostable xylanase from *Thermoascus
aurantiacus*. J. Mol. Biol., 288, 999-1012.
<https://www.ncbi.nlm.nih.gov/pubmed/10329194>
and then updated the 1.8 A crystal structure derived sequence with ultra
high resolution electron density map. We were also able to model
additional terminal residues in ultra high resolution map (reported in 2003
paper below).
2) Natesh R, Manikandan K, Bhanumoorthy P, Viswamitra MA, Ramakumar S.
(2003). Thermostable xylanase from *Thermoascus aurantiacus* at ultrahigh
resolution (0.89 Å) at 100 K and atomic resolution (1.11 Å) at 293 K
refined anisotropically to small-molecule accuracy. Acta Crystallogr., D
59,105-117. <https://www.ncbi.nlm.nih.gov/pubmed/12499546>
I will be able happy to help off the egroup if you need more
information/help.
Best regards,
Natesh
--
----------------------------------------------------------
"Live Simply and do Serious Things .. "
- Dorothy Mary Crowfoot Hodgkin OM, FRS
"In Science truth always wins"
- Max Ferdinand Perutz OM FRS
----------------------------------------------------------
Dr. Ramanathan Natesh
Assistant Professor,
Founding President and President Cryo Electron Microscopy and 3 Dimensional
Image Processing Society of India (CEM3DIPSI)
http://cem3dipsi.iisertvm.ac.in/
School of Biology,
Indian Institute of Science Education and Research Thiruvananthapuram
(IISER-TVM),
Maruthamala P.O., Vithura,
Thiruvananthapuram, 695551, Kerala, India
nat...@iisertvm.ac.in
http://faculty.iisertvm.ac.in/natesh
*Researcher ID*: http://www.researcherid.com/rid/C-4488-2008
*ORCID*: http://orcid.org/0000-0002-1145-5962
Vidwan-ID : 94134: http://iisertvm.irins.org/profile/94134
*PUBLONS*: https://publons.com/author/1520837/ramanathan-natesh#profile
Office Ph. 0091- 471-2778087
On Fri, 29 Jul 2022 at 15:45, Jon Cooper <
0000488a26d62010-dmarc-requ...@jiscmail.ac.uk> wrote:
> Hello, I am looking for suggestions of ways to check a 1.7 Angstrom X-ray
> sequence for a protein where it is impractical to do experimental
> sequencing, protein or DNA. The structure refines to publishable R/R-free
> and the main ambiguities seem to be Thr/Val, Asp/Asn and Glu/Gln where
> alternative H-bonding networks are possible. Running alpha-fold seems an
> interesting option? Any suggestions much appreciated.
>
> Cheers, Jon.C.
>
> Sent from ProtonMail mobile
>
>
>
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