Thanks for your input. To avoid precipitation what about doing something like this? For example, as we know: AlCl3 + 3NaF = AlF3 + 3NaCl
Therefore, adding 100mM AlCl3 plus 300mM NaF to get 100mM of AlF3 solution, then directly using this 100 mM stock of AlF3 for the final dilution, say 1 or 2 mM in the reaction mix ? Or it is recommended to use AlCl3 and NaF hoping to form AlF3 in the reaction mix. Best Faisal On Tue, Jun 21, 2022 at 2:08 PM Guillaume Gaullier < guillaume.gaull...@univ-tlse3.fr> wrote: > Hi Firdous, > > I have read a fair amount of papers about ATP-dependent chromatin > remodelers in which ADP-BeF3 is often cited. It is made as follows (quoting > from one of these papers): "ADP-BeF3 was prepared as a 10x stock (10 mM > ADP, 30 mM BeSO4, 150 mM NaF, 10 mM MgCl2), freshly before use." > > So the trick is to get all you need from a mixture of soluble compounds, > instead of from only one poorly soluble salt. Not sure this will work the > same with Al instead of Be, but it’s easy enough to try: use any > known-soluble salt of Al instead of this BeSO4, and see if it works. NaF is > extremely toxic, so use adequate protective measures (PPE, hood, etc.). > > Good luck, > > Guillaume > > > On 21 Jun 2022, at 19:38, Firdous Tarique <kahkashantari...@gmail.com> > wrote: > > Hi > > Sorry for the off topic question but can anybody please suggest how to > dissolve Aluminium Fluoride in water ? I know it has little solubility in > water but have seen people using AF3 for their structural studies (ADP + > AF3 as ATP analogue). Is there any other solvent where they have made AF3 > solution or is there some trick to dissolve it in water?. > > Thanks > > Firdous > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
