Hi Sarah, Thank you for that great summary! I agree, there are different uses for each instrument. We do enjoy our Gryphon for screening, though I find the dead volume for protein dispense to be very large and generally unreliable. I have heard a lot of great feedback about the Oryx from folks and it may serve us well as we need a system for focused optimization screens and not necessarily the high throughput one might need at your institute! The downside is not having humidification and the system is a bit slow. NT8 also sounds like a great instrument, though some folks have mentioned it experiences frequent mechanical failure resulting in costly repair bills with equally costly service contracts.
Anyway, I much appreciate your input! The crystallography community is so unique in that there are always folks like you and Eddie willing to lend opinions or help - I love it! All my best, Shawn Rumrill Chemistry and Chemical Biology, The Lab of Dr. Eddy Arnold Center for Advanced Biotechnology and Medicine Rutgers University - New Brunswick shawn.rumr...@rutgers.edu ________________________________ From: Sarah Bowman <sbow...@hwi.buffalo.edu> Sent: Monday, May 23, 2022 1:49 PM To: Shawn Rumrill <shawn.rumr...@rutgers.edu>; Edward Snell <esn...@hwi.buffalo.edu>; CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: Re: [ccp4bb] Crystallization Robots Hi Shawn, Thanks for your email. As Eddie mentioned, we have a lot of robotics & liquid handling instruments at the National High-Throughput Crystallization (HTX) Center at HWI. For the high-throughput 1536 micro assay plates we set up, we use the Art Robbins Gryphon with 384 syringe head and PlateMates (the PMs are not available any longer that I know of). For optimization and scale up, we currently use both the Formulatrix NT8 and the Gryphon with 96 syringe head. We are in the process of ordering a Mosquito, and do have an Oryx4 (but don't use that frequently because of the high-throughput nature of the set-ups in the HTX Center). They all have different pros and cons, depending on your needs... but we do really like the humidity controlled option on the NT8. We use the NT8 for vapor diffusion, microbatch under oil, LCP, and seeding, so it can really handle a lot of different configurations for set-up. Definitely feel free to reach out to us (www.getacrystal.org<https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.getacrystal.org%2F&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C8aa6dd83d2dd4e0f337c08da3ce49443%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889250307005594%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=jVgqW5LFklU6IsfM6B5OdvzdVRk2vSWe%2FR4J8g8z618%3D&reserved=0>) if you need any assistance with crystallization! Hope that helps! Warmly, Sarah Sarah EJ Bowman PhD Director | National High-Throughput Crystallization Center Associate Research Scientist | Hauptman-Woodward Medical Research Institute Research Associate Professor | Department of Biochemistry | University at Buffalo p: +1 716 898 8623 e: sbowman at hwi.buffalo.edu Research Webpage<https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fhwi.buffalo.edu%2Fscientist-directory%2Fsbowman%2F&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C8aa6dd83d2dd4e0f337c08da3ce49443%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889250307005594%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=OhTJrP8WsOHohvW7N3egwOvCt51d5Ou2Dvw1h7C5F%2BY%3D&reserved=0> Crystallization Center Webpage<https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.getacrystal.org%2F&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C8aa6dd83d2dd4e0f337c08da3ce49443%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889250307005594%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=jVgqW5LFklU6IsfM6B5OdvzdVRk2vSWe%2FR4J8g8z618%3D&reserved=0> Hauptman-Woodward Medical Research Institute 700 Ellicott Street | Buffalo, NY 14203 From: Shawn Rumrill <shawn.rumr...@rutgers.edu> Date: Monday, May 23, 2022 at 12:45 PM To: Edward Snell <esn...@hwi.buffalo.edu>, "CCP4BB@JISCMAIL.AC.UK" <CCP4BB@JISCMAIL.AC.UK> Cc: Sarah Bowman <sbow...@hwi.buffalo.edu> Subject: Re: [ccp4bb] Crystallization Robots Hi Eddie and Sarah, Thank you so much for your insights! I look forward to hearing about the NT8. From the responses I've been getting, it sounds like the Oryx does many things well, albeit a bit more This sender is trusted. sophospsmartbannerend Hi Eddie and Sarah, Thank you so much for your insights! I look forward to hearing about the NT8. From the responses I've been getting, it sounds like the Oryx does many things well, albeit a bit more slowly. Oryx also has a very reasonable price point. Much appreciate the resources. I am always impressed by the work you guys are doing at the National Crystallization Center. Hope to one day give it a try! Cheers, Shawn Rumrill Chemistry and Chemical Biology, The Lab of Dr. Eddy Arnold Center for Advanced Biotechnology and Medicine Rutgers University - New Brunswick shawn.rumr...@rutgers.edu ________________________________ From: Edward Snell <esn...@hwi.buffalo.edu> Sent: Monday, May 23, 2022 10:33 AM To: Shawn Rumrill <shawn.rumr...@rutgers.edu>; CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Cc: Sarah Bowman <sbow...@hwi.buffalo.edu> Subject: RE: [ccp4bb] Crystallization Robots Dear Shawn, I do know that the National Crystallization Center has recently installed an NT8 and we also have an Oryx4 - not quite the same comparison. Sarah Bowman who directs the Center (cc'd) can probably let you know how the NT8 is working. The Center has a lot of expertise with testing many different chemicals. The Oryx4 is very capable but that is used in a lower throughput setting for just a few labs rather than the number of samples planned for the NT8. Details on the National Crystallization Center are available at https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fhwi.buffalo.edu%2Fhigh-throughput-crystallization-center%2F&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C1360cda25fe1404f693808da3cc941f2%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889132450783990%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=ieD1MpTQbaJukC8ENsGPum68%2FGcfouHrpiE53AGWkzY%3D&reserved=0<https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fhwi.buffalo.edu%2Fhigh-throughput-crystallization-center%2F&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C8aa6dd83d2dd4e0f337c08da3ce49443%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889250307005594%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=7cWGoPAxSVdWj6yzJvFb%2FhTTVbpfGWIkV6MXNluj5Hg%3D&reserved=0>. It is a Center that provides access to a number of crystallization instruments, software, and expertise. If you want to remember it easily it can also be found at 'getacrystal.org' The paper describing it is very outdated and we hope to have an updated publication in the near future to celebrate over two decades of operations. Best, Eddie Edward Snell Ph.D. President and CEO | Hauptman-Woodward Medical Research Institute Director | NSF BioXFEL Science and Technology Center Professor, Materials Design and Innovation | University at Buffalo, SUNY p: +1 716 898 8631 | f: +1 716 898 8660 e: esn...@hwi.buffalo.edu skype: eddie.snell Hauptman-Woodward Medical Research Institute 700 Ellicott Street | Buffalo, NY 14203-1102 hwi.buffalo.edu -----Original Message----- From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Shawn Rumrill Sent: Monday, May 23, 2022 10:02 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Crystallization Robots Hello, Does anyone have experience with either the Oryx8 or Formulatrix NT8 for setting up crystallization trays? Both instruments look very capable on paper. I am looking for something that is easy to use, robust, and can handle a variety of different crystallization experiments (screening and optimization). What do you like/dislike about either system? Thanks for your insights! Shawn ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCCP4BB%26A%3D1&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C1360cda25fe1404f693808da3cc941f2%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889132450783990%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=NQEXORmbCzvSggXUTBeyWLl5zW4meN7tabRe2c8AQN0%3D&reserved=0<https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCCP4BB%26A%3D1&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C8aa6dd83d2dd4e0f337c08da3ce49443%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889250307005594%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=79xJk3UoJ5vXHYgApTyPbIWnuRK72huz4CIX5vN%2BLcI%3D&reserved=0> This message was issued to members of https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.jiscmail.ac.uk%2FCCP4BB&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C1360cda25fe1404f693808da3cc941f2%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889132450783990%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=VcHB3FnI65wjhAPcHhQAhkx%2FMqcZPDH%2B%2FXLdWgcZGMI%3D&reserved=0<https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.jiscmail.ac.uk%2FCCP4BB&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C8aa6dd83d2dd4e0f337c08da3ce49443%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889250307005594%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=xE0wG42m8kithM4ttkN7IpatXxGEnJ6ipiZGWopY%2BU4%3D&reserved=0>, a mailing list hosted by https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.jiscmail.ac.uk%2F&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C1360cda25fe1404f693808da3cc941f2%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889132450783990%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=Djk%2Bk%2FjlhnlnmohPna3C9THggQhY92%2FyZbQOH1qXLL8%3D&reserved=0<https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.jiscmail.ac.uk%2F&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C8aa6dd83d2dd4e0f337c08da3ce49443%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889250307005594%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=DCQXv1zklya0bNwPRmdmvYxMB3dH6ZEB%2FkAU8thz62Q%3D&reserved=0>, terms & conditions are available at https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fpolicyandsecurity%2F&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C1360cda25fe1404f693808da3cc941f2%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889132450783990%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=YuOrJygePymLVgxK%2FvU3L9NykDjIgfJNBuNZYVx283I%3D&reserved=0<https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fpolicyandsecurity%2F&data=05%7C01%7Cshawn.rumrill%40rutgers.edu%7C8aa6dd83d2dd4e0f337c08da3ce49443%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C637889250307005594%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=BOnj9eTowTUm3H0GImyYlfqDbfhEtrs4romUBAhpixw%3D&reserved=0> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
