MES or HEPES, I reckon. Both of them can lose definition for the electron density in the morpolino/piperazine 6-membered ring due to flexibility between the ring and the sulphonic acid group when - leading to the tadpole-like density you describe.
HTH, Dave -- Dr David C. Briggs CSci Senior Laboratory Research Scientist Signalling and Structural Biology Lab The Francis Crick Institute London, UK == Diamond User Group (DUC) MX representative == about.me/david_briggs ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Xavier Brazzolotto <xbrazzolo...@gmail.com> Sent: 13 March 2022 17:28 To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: Re: [ccp4bb] mysterious density External Sender: Use caution. A MES buffer molecule ? > Le 13 mars 2022 à 18:08, doriano lamba <doriano.la...@ic.cnr.it> a écrit : > > Glycerol-3-phosphate? > My 2cents > DL > Il 2022-03-13 15:19 Amir Khan ha scritto: >> Hi, >> I wonder whether anyone can advise on the ‘tadpole’ like density. >> The head is currently a phosphate >> and I’ve placed a water in tail, but it obviously looks connected… >> would appreciate any help! >> Green density at 5 sigma (Fo-fc), while blue is 2fo-fc at 1.3 sigma. >> Crystallization condition is unknown, though it came from sparse >> matrix commercial (either Wizard, Pact Prem, or JCSG+). >> Thanks! >> Amir >> ------------------------- >> To unsubscribe from the CCP4BB list, click the following link: >> 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