One could use PISA to get a rough distribution of oligomerization states of proteins in the PDB and compare bacterial vs mammalian... there always will be a question though of whether any bias is inherent in proteins or driven by crystallizability itself.
Personally, I always though that bacterial proteins crystallize better because they express better and are more stable. All of this is hand waiving on my part, of course. --- I don't know why the sacrifice didn't work. The science seemed so solid. Julien XIII, Lord of the Lemurs On Fri, Nov 12, 2021 at 9:52 AM Frank von Delft <frank.vonde...@cmd.ox.ac.uk> wrote: > Hello all > > Two decades ago, I remember (!) much talk about a reason that bacterial > proteins crystallize "more easily" is that they tend to come as > oligomers (dimers and up), and that this internal symmetry made them > happier to crystallize. > > Did anybody ever publish hard evidence? Or even, is there a primary > citation for the idea? > > Thanks > Frank > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
