It may also be possible to separate the different phosphorylated forms on an isoelectric focusing gel. Best, Herman
Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> Im Auftrag von Patrick Loll Gesendet: Mittwoch, 15. September 2021 15:12 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] Phosphatase enzymatic assay Phos-tag gels are an option (albeit a fiddly one). Cheers, Pat Loll ___________________________ Patrick J. Loll, PhD (he, him, his) Professor of Biochemistry & Molecular Biology Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St. Philadelphia, PA 19102-1192 USA (267) 359-2653 pj...@drexel.edu<mailto:pj...@drexel.edu> On 15 Sep 2021, at 8:28 AM, Andrea Moretti <andrea.more...@unige.ch<mailto:andrea.more...@unige.ch>> wrote: Dear CCP4 community, sorry for the off topic question but I am struggling with enzymatic assays and I would need your help. I am trying to measure dephosphorylation of a phosphorylated kinase by a ser/thr phosphatase. So far I tried to measure it by malachite green assay, anti pSer/pThr antibody and PNPase coupled assay but nothing really worked for me. NMR is unfortunately not an option since I can't make enough of the proteins. So any hints on phosphatase assays would be helpful. Thanks! Best wishes, Andrea -- Andrea Moretti Structural Plant Biology Laboratory Department of Botany and Plant Biology Sciences III University of Geneva 30 Quai E. Ansermet 1211 Geneva Switzerland Email: andrea.more...@unige.ch<mailto:andrea.more...@unige.ch> Phone: +41 22 379 3029 (office) web:http://structplantbio.org<http://structplantbio.org> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> This message was issued to members of www.jiscmail.ac.uk/CCP4BB<http://www.jiscmail.ac.uk/CCP4BB>, a mailing list hosted by www.jiscmail.ac.uk<http://www.jiscmail.ac.uk>, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/<https://www.jiscmail.ac.uk/policyandsecurity/> Patrick Loll pjl...@gmail.com<mailto:pjl...@gmail.com> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
