Hello, do you start with a loop and coot/refmac give you back a loop or do you start with a strand and get a loop back? Which fits the density best and how good is the initial fit? Are there any extra restraints which always make it loop back on itself when it shouldn't ;-?
Cheers, Jon.C. Sent from ProtonMail mobile -------- Original Message -------- On 11 Aug 2021, 17:49, Matthew Snee wrote: > Is it not just a case of adding "beta sheet restraints" in the real-space > refine options menu? > > If it goes back to a loop during refinement then that may mean that the > atomic positions don't meet the requirements? > > Get [Outlook for Android](https://aka.ms/AAb9ysg) > --------------------------------------------------------------- > > From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of rohit kumar > <rohit...@gmail.com> > Sent: Wednesday, August 11, 2021 5:41:55 PM > To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> > Subject: [ccp4bb] convert loop into a sheet > > Hello Everyone, > > I was trying to build my peptide (8 AA long) as ligand in coot. Peptide > should be in the form of Sheet, after multiple cycles of refinement peptide > still in the loop form. If anyone could help me, how to convert this loop > structure into a sheet in coot , would be very helpful? > I am using ccp4-Refmac5 for the refinement. > > Thank you > Rohit > > -- > > Regards > Dr. Rohit Kumar Singh > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
