Could be 5 water molecules. Pentagons of water molecules are common in high resolution structures. Add 5 waters and see if the inter-water distances are appropriate for hydrogen bonding (2.5-3.2 A).
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Sam Tang <samtys0...@gmail.com> Date: Monday, March 22, 2021 at 9:00 AM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] unknown density WARNING: This message has originated from an External Source. This may be a phishing expedition that can result in unauthorized access to our IT System. Please use proper judgment and caution when opening attachments, clicking links, or responding to this email. Hello fellow colleagues Hope you are all well while the pandemics persists. I just wonder if anyone may have an idea what this density (looking like a pentagon) might be. The data was collected to 1.8 A and crystal was grown in Bis-tris + PEG3350. Imidazole residual? Nucleotide (the protein itself is nucleotide-binding, but shouldn't be at this particular site)? https://drive.google.com/file/d/1L9UBFmW72P214itM2HJR_DVy3FaA6FEZ/view?usp=sharing<https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdrive.google.com%2Ffile%2Fd%2F1L9UBFmW72P214itM2HJR_DVy3FaA6FEZ%2Fview%3Fusp%3Dsharing&data=04%7C01%7C%7Ca3b0c198e3e14bd9405408d8ed3acf6a%7Ce3fefdbef7e9401ba51a355e01b05a89%7C0%7C0%7C637520184290892971%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=2kZEUkgOzddNoFf57Rb0ZBj8CwmamIuYxqoo1BWrVMM%3D&reserved=0> Thanks! BRS Sam ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCCP4BB%26A%3D1&data=04%7C01%7C%7Ca3b0c198e3e14bd9405408d8ed3acf6a%7Ce3fefdbef7e9401ba51a355e01b05a89%7C0%7C0%7C637520184290902965%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=kUM5lnFwUIBPz2fK6eHFi90KFna%2B16R1ffFI0GiNgIY%3D&reserved=0> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
