For us, a C-terminal macro domain tag worked well to crystallize a
rather flexible target protein.
https://pubmed.ncbi.nlm.nih.gov/27774698/
best regards,
Michael
On 15.03.21 16:51, Pascal Egea wrote:
Hi Herman,
I will add a few points to all the excellent advice already provided
to you.
If you decide to try this option of crystallizing a fusion protein of
your target, I would consider using N-terminal but also C-terminal
fusions. We have had success using MBP in N-ter and the superfolder
GFP in C-terminal for a few 'vexing' proteins from /Plasmodium
falciparum. /It helped us either to obtain crystals/structures (as you
seek) but also provided a way to circumvent a tendency for twinning
from our target in some specific cases.
GFP has the added benefit that your fusion crystals should be
bright yellow so that speeds up the screening process a bit.
I hope this helps. Good luck
Best regards,
Pascal Egea, PhD
UCLA School of Medicine
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