On 08/02/2021 13:45, Afshan Begum wrote:
I have a trouble to fix the lysin modified residue in to the PDB coordinate.
what i did,
I get the KCX(Modified lysin) coordinate from monomer library in COOT (0.9) , merge in to the coordinate
file , its listed into position 193 lysin then run rafmac 5 , its place the residue but as unconnected and
produce its own number.
In the second step
I take the kcx coordinate in the text pdb file and place into between 192 and 194 and change reside number
193 . Save it.
Now it can not be read for further refinement.
Could you people suggest me how to fix it?
If I read you right, this is not the most straightforward procedure.
While centred on the lysine:
Calculate -> Modelling -> Replace Residue -> KCX -> Mutate
Now give it a bit of RSR... and you're done.
Paul.
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