Well - you dont give the resolution of your data or the "wilson B" which will be recorded in the data processing log. If the resolution is 3A or less a) it is hard to refine a B value, and b) it certainly should be high..
So more information is needed. Eleanor On Mon, 4 Jan 2021 at 14:39, Silvia Napolitano < silvia.napolit...@mol.biol.ethz.ch> wrote: > Dear CCP4 Community, > I am currently working on the structure of a monomeric protein of 23KDa. > The protein is generally quite "loopy" and I think I am mid-way refinement. > At the moment I am struggling, among other things, with an extremely high > B-factor and I'm not sure how to lower it. > Do you have any suggestions on how I can proceed to obtain a lower average > B-factor? (I am sorry if the question is rather naive, but I have worked > very little on X-ray structures so far) > I attached a screenshot of the polygon obtained from the last refinement > (the geometry now should be a bit improved, I am running a new job at the > moment). I will be happy to provide further files if needed. > Many thanks in advance for your precious help! > I wish you a nice start to the new year. > Best > Silvia > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
