Hi Jon, The goniometer on a TEM microscope typically cannot rotate the full 360 degrees and therefore we can't ramp it up to speed before initiating collection and slow it down after collection has ended as is typically done for X-ray crystallography. This causes the tilt speed and therefore the oscillation per frame to be less reliable in the early and late frames. We added a small delay between starting the stage tilt and exposing the crystal/collecting the first image to help with this.
Best regards, Jessica On Wed, Oct 21, 2020 at 2:30 PM Jon Cooper < 0000488a26d62010-dmarc-requ...@jiscmail.ac.uk> wrote: > Hello, I am interested to know why "it is conventional to delete/omit > first/last image". Forgive my ignorance. > > Best wishes, Jon Cooper. jon.b.coo...@protonmail.com > > > > > > -------- Original Message -------- > On 21 Oct 2020, 19:23, Doo Nam Kim < > 00004e720d49e642-dmarc-requ...@jiscmail.ac.uk> wrote: > > > Dear all, > > What I knew is starting angle (-60) and ending angle (+60). > Additionally, I know number of image files generated by mrc2smv is 120 > from my mrc files. > > Since it is conventional to delete/omit first/last image, > > changing into a decently "accurate" approximation as > > STARTING_ANGLE=-60 > DATA_RANGE=2 119 > OSCILLATION_RANGE=1 > > reproduced my predecessor's completeness. > > Thank you all > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > -- Jessica Bruhn, Ph.D Principal Scientist NanoImaging Services, Inc. 4940 Carroll Canyon Road, Suite 115 San Diego, CA 92121 Phone #: (888) 675-8261 www.nanoimagingservices.com ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
