Hi Bernhard, The Department has an Avestin emusiflex here, and it is kept in the cold room. Works great as long as your cells are properly resuspended and in a big enough volume. Highly recommended.
Adam On Sun, Aug 16, 2020 at 4:48 PM Pascal Egea < 00004aa44fc90f38-dmarc-requ...@jiscmail.ac.uk> wrote: > Dear Bernhard , > I would recommend the emulsified from Avestin. It is great . Depending on > your budget you can ask for stainless steel (probe to slow tear ) or > ceramic (longer lasting but a bit more expensive). > We have the ceramic but I have worked with the stainless steel one and it > is really good with E. coli cells. Not the best for > Yeast despite pressurizing more. Yeast are better dealt with bead beaters > or cryo-mill grinders. > 3 passes are usually sufficient to process up to 250 ml of extract in a > decent time (15-20) minutes . > > We operate ours at room temperature just cool the serpentine tubing coming > out of the chamber in ice . There is an option to add a cooling plate but > we have never considered it. Overheating comes from overpressurizing but > for E Coli at 15000 psi max 3 passes will do the trick without overhea tu > bf the sample. I have processEs successfully many membrane proteins And > protein complexes with it. > > I have never seen one in a cold room to be honest but it should be fine > although I don’t think it would be necessary . I would Ask avestin about > that . > Maintenance and cleaning is simple . Once in a while I will replace the > seal, it s not very difficult to do. The whole assembly is metal so you can > sterilize the whole thing After complete disassembly in extreme cases . > This is a bit more involved but not overwhelming. > The people at Avestin are super nice and responsive ( Canadians) so they > will guide you if necessary . And we have had their engineers show up > sometimes to just check the instrument for free . I have had this one for > 10 years in my lab now. And before that was using one for 7 years during my > post doc. I would not lyse bacteria by any other method now. > > I hope this helps. > All the best. > Pascal > > On Sat, Aug 15, 2020 at 9:08 PM Bernhard Lechtenberg < > lechtenber...@wehi.edu.au> wrote: > >> Dear colleagues, >> >> >> >> We are currently looking to purchase a cell disruptor/homogeniser mainly >> for routinely processing a few 100 mls of E. coli suspensions. With the >> current COVID-19 restrictions it is very difficult for us to test any >> equipment. I thus hope that some of you can share their experiences with >> the different models. I found a similar thread on the CCP4BB from 2013 but >> wondered if anybody had had some more up to date information. >> >> >> >> We are mainly looking at the Avestin Emulsiflex C3 homogeniser and the >> Microfluidics LM20 Microfluidizer. In particular we are interested to know >> more about ease of use, maintenance, reliability and if anybody operates >> these in a cold room (4°C). >> >> >> >> Thanks in advance, >> >> >> >> Bernhard >> >> >> >> <https://www.google.com/maps/search/1G+Royal+Parade+%0D%0A+Parkville+VIC+3052+%0D%0A+Australia?entry=gmail&source=g> >> >> >> >> <https://www.google.com/maps/search/1G+Royal+Parade+%0D%0A+Parkville+VIC+3052+%0D%0A+Australia?entry=gmail&source=g> >> >> -- >> >> Bernhard C. Lechtenberg, Ph.D. >> >> Laboratory Head >> >> Ubiquitin Signalling Division >> >> The Walter and Eliza Hall Institute >> >> 1G Royal Parade >> <https://www.google.com/maps/search/1G+Royal+Parade+%0D%0A+Parkville+VIC+3052+%0D%0A+Australia?entry=gmail&source=g> >> >> Parkville VIC 3052 >> <https://www.google.com/maps/search/1G+Royal+Parade+%0D%0A+Parkville+VIC+3052+%0D%0A+Australia?entry=gmail&source=g> >> >> Australia >> <https://www.google.com/maps/search/1G+Royal+Parade+%0D%0A+Parkville+VIC+3052+%0D%0A+Australia?entry=gmail&source=g> >> >> Phone: +61 3 9345 2217 >> >> Email: lechtenber...@wehi.edu.au >> >> >> >> _______________________________________________ >> >> The information in this email is confidential and intended solely for the >> addressee. >> You must not disclose, forward, print or use it without the permission of >> the sender. >> >> The Walter and Eliza Hall Institute acknowledges the Wurundjeri people of >> the Kulin >> Nation as the traditional owners of the land where our campuses are >> located and >> the continuing connection to country and community. >> _______________________________________________ >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > -- > Pascal F. Egea, PhD > Associate Project Scientist > UCLA, David Geffen School of Medicine > Department of Biological Chemistry > Boyer Hall room 356 > 611 Charles E Young Drive East > Los Angeles CA 90095 > office (310)-983-3515 > lab (310)-983-3516 > email pegea at mednet.ucla.edu > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > -- Adam Middleton, Ph.D. Research Fellow Department of Biochemistry School of Biomedical Sciences University of Otago 710 Cumberland St. Dunedin 9016 New Zealand adam.middle...@otago.ac.nz ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
