Sometimes ghost like this mean there is a spacegroup error - absences can be the result of the non-crystallographic translation and not be truly indicitive of the spacegroup. what is the possible spacegroup and what is the NC translation vector? Eleanor
On Wed, 29 Jul 2020 at 14:13, Schreuder, Herman /DE < [email protected]> wrote: > Dear Rafal, > > At this resolution, one sees many amino-acid side chains with alternative > conformation, so it might be a good idea to test if this is also true for > nucleotides. > > Best, Herman > > -----Ursprüngliche Nachricht----- > Von: CCP4 bulletin board <[email protected]> Im Auftrag von Rafal > Dolot > Gesendet: Mittwoch, 29. Juli 2020 14:42 > An: [email protected] > Betreff: [EXTERNAL] [ccp4bb] Problems with refinement of nucleic acid > structure > > EXTERNAL : Real sender is [email protected] > > > > Dear CCP4 users, > > I'm working on the another nucleic acid duplex structure. After several > stages of rebuilding and refinement I observed something like a "shadow" > of the second duplex position. Have you any ideas to explain this > phenomena? Should I place the second molecule with occupancy completed to > the first one? Or maybe is it a kind of disorder in crystal lattice? > Data were collected to 1.02A using synchrotron radiation (processed with > XDS), but I have another datasets collected to lower resolution (from > Zn-SAD) with visible similar effects. Refinement was done using Refmac > (R/Rfree 21.5/24.0). Data not appeared to be twinned, but Xtriage detected > pseudo-translational symmetry. > > Thank you for any advices. > > Rafal > > |----------------------------------------------| > |Rafal Dolot, Ph.D. | > | | > |Polish Academy of Sciences | > |Centre of Molecular and Macromolecular Studies| > |Department of Bioorganic Chemistry | > |Macromolecular Crystallography Team | > |Sienkiewicza 112 | > |90-363 Lodz, Poland | > |Phone: +48(42)6803215 | > |Cell: +48 502897781 | > |----------------------------------------------| > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > > https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_WA-2DJISC.exe-3FSUBED1-3DCCP4BB-26A-3D1&d=DwICAg&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=cDOmaUoERSX6Rz_GQvd1VxncHBGPvEnDlYJSypA0P-M&s=bxs7KYSqKDM2UBAguUYP_9utSJpoMdJ0N3qRJOBQ1v0&e= > > This message was issued to members of > https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_CCP4BB&d=DwICAg&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=cDOmaUoERSX6Rz_GQvd1VxncHBGPvEnDlYJSypA0P-M&s=dJtlr7D5BN24QQ-TBSNysQi31jIrarcnXWm4TpREHf0&e=, > a mailing list hosted by > https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk&d=DwICAg&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=cDOmaUoERSX6Rz_GQvd1VxncHBGPvEnDlYJSypA0P-M&s=61QRZ35Nd2knlHLbWpFLGGUD_aa18yIOsQtLlDQwcXo&e=, > terms & conditions are available at > https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_policyandsecurity_&d=DwICAg&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=cDOmaUoERSX6Rz_GQvd1VxncHBGPvEnDlYJSypA0P-M&s=sNhiuaGAxt8e9Fhizzcy4JHfAFv4fAW-8tUFxrYZDmk&e= > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
