Hello, do you have any negative difference density for the current position of the His side chain? If so, it may be in two confirmations. Just a thought.
Best wishes, Jon Cooper, E-mail: jon.b.coo...@protonmail.com -------- Original Message -------- On 20 Jul 2020, 17:16, samer halabi wrote: > Hello all, > I have few blobs in an MHC II structure I am working on, especially opposite > to Histidine as in the accompanying screenshot, that I am confused about. > > In the crystal conditions, I have Tris, Imidazole, Acetate, PEG and Glycerol. > Whatever ligand I am fitting in I am getting a clash (overlap -1.029), which > makes me think whether there is a covalent bond forming between Histidine and > other molecule. Perhaps by oxidation. > > I would greatly appreciate if you can advice me about it, whether there is > some kind of ligand I can try to fit and if this is something that occurs in > some structures. > Thank you. > Best regards, > Samer > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
