Hi Joao The top of the page was cut off in your screenshot so I can't see the command line you typed. It would be better to copy/paste the entire text, including the input command line and the output, into the email rather than taking a screenshot. 'Cannot open file' most likely means that the input filename was not typed correctly or the path was not specified correctly if it's in another directory. Anyway you really shouldn't be calling the MTZFIX or EDSTATS programs directly. Using the Perl script 'edstats.pl' http://www.ccp4.ac.uk/dist/checkout/edstats/edstats.pl is much easier. It should be in your path after running the CCP4 setup script.
Cheers -- Ian On Mon, 15 Jun 2020 at 18:41, Joao Ramos <jc.ra...@campus.fct.unl.pt> wrote: > Dear Ian, > > Thanks for the reply! > > I've sourced the setup script, but now I get a 'cannot open file' error > message (screenshot attached) when trying to run MTZFIX. > > Best regards, > Joao Ramos > > Ian Tickle <ianj...@gmail.com> escreveu no dia segunda, 15/06/2020 à(s) > 16:57: > >> >> Hi Joao >> >> For all CCP4 programs you need to source your CCP4 setup script. >> >> Cheers >> >> -- Ian >> >> >> On Mon, 15 Jun 2020 at 15:29, Joao Ramos <jc.ra...@campus.fct.unl.pt> >> wrote: >> >>> Dear CCP4bb, >>> >>> I'm trying to obtain RSC metrics using the CCP4 program EDSTATS for a >>> Phenix refinement job, in order to have a more reliable analysis of model >>> quality. From what I read, one should go through the program MTZFIX and >>> then create the FFT maps (density and difference maps). However, while >>> attempting to run the Phenix output mtz file in MTZFIX, I receive an error >>> message saying: "Cannot open environ.def". >>> >>> My questions are: >>> 1) Is the way to go from the Phenix refinement output files to EDSTATS >>> described earlier correct? Are there alternative programs included in >>> Phenix? >>> 2) How to fix the error and run MTZFIX? >>> >>> I'm currently using the new ccp4 v7.1, in macOS v10.14.5. >>> >>> Best regards, >>> Joao Ramos >>> >>> ------------------------------ >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/wa.exe?SUBED1=CCP4BB&A=1 >>> >> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
