Hmm - this finally was rather deprecated - there are some less happy consequences to have all reflections in a particular resolution shell excluded. If your NCS means there is pseudo- symmetry in the diffraction and it would be possible to have a cell with higher point symmetry (an example might be be Pmmm or P4mm if a(Pmmm) ~ b(Pmmmm). ) then you could assign freer flags in the higher symmetry group and extend then to the lower...
What is your cell, and do you know the NCS operators? Eleanor On Mon, 15 Jun 2020 at 16:54, <Shymaa Damfo> <shymaa.damfo...@ucl.ac.uk> wrote: > Hello, > > I'm refining protein structure in Refmac and I have NCS greater than 4 ( > I've 8 identical subunits in the asymmetric unit). > > In the "Refinement parameters" section in Refmac, how can I pick Rfree set > based on thin resolution shells rather than in the normal random method? > > I've read about the program "dataman" from the usf suite which could help > with this but found it very complicated. > > Do you have any advice to solve this? or would you recommend any other > program? > > > Thank you in advance > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
