Thank you all, I have done it with chimera. It does structural alignment and use it as a template. Then, you can add sequences one after the other and align to this template. You can save this alignment in clustalw format and load it to ESPrit3 and do a nice picture. Armando
> El 8 abr 2020, a las 19:22, Guillaume Gaullier <guilla...@gaullier.org> > escribió: > > Hi Armando, > > This seems doable with ChimeraX: https://www.cgl.ucsf.edu/chimerax/ > <https://www.cgl.ucsf.edu/chimerax/> > > More specifically, its matchmaker command will align two structures and print > the corresponding sequence alignment: > https://www.cgl.ucsf.edu/chimerax/docs/user/commands/matchmaker.html > <https://www.cgl.ucsf.edu/chimerax/docs/user/commands/matchmaker.html> > You can then save the sequence alignment to a file, and use it in your > favorite sequence alignment program along with the sequences for which you > don’t have a structure. > > This is one option among many, as pretty much every structure visualization > program can superimpose two similar structures (but I don’t know how many of > them make it as easy to save the sequence alignment). > > Hope this helps, > > Guillaume > > >> On 8 Apr 2020, at 19:04, Armando Albert <xalb...@iqfr.csic.es >> <mailto:xalb...@iqfr.csic.es>> wrote: >> >> Dear all, >> I want to align two structures and then, I want to align several sequences >> to that structural alignment. >> How can I do this? >> Armando >> >> ######################################################################## >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
