Dear James, On Fri, Mar 20, 2020 at 03:59:01PM -0700, James Holton wrote: > ORF8 has only one homolog in the PDB: 5o32 with 25% identity over a stretch > of 60 residues. This homologous region contains the S84L site (Val I544 in > 5o32). I had a quick look and appears to be a cavity-filling mutation to > me. Not very big, but maybe something could fit in there. To be sure we'd > need a structure of ORF8.
This is a very good example of why we all are trying to get raw diffraction data deposited as soon as possible to the publication and deposition of a structure. It highlights the potential benefit of revisting some important structures right at the level of the raw data. The paper says Diffraction data were collected at European Synchrotron Radiation Facility (ESRF) and Swiss Light Source at Paul Scherrer Institute (SLS-PSI). Diffraction data were processed by XDS and AIMLESS. Crystals of C3b–miniFH–FI exhibited space group P1 and diffracted to a resolution of 3.8 Å. However, the diffraction data was highly anisotropic. The data were therefore truncated to 4.2-Å resolution and rescaled using the Diffraction Anisotropy Server. Obviously, that was state-of-the-art in 2015 (data collection) and 2017 (publication) - but maybe new methods could indeed do better on this already existing structure if the raw data became available. Maybe nothing will change in terms of structure quality and (most importantly) structure interpretation ... who knows. We've contacted the authors - who were very quick and extremely helpful in providing us with raw images for some other deposited project from their lab a few weeks ago, so expect and hope for a similar response when/if the data can be located. > Good luck to you all, and stay healthy. Ditto! Cheers Clemens -- *-------------------------------------------------------------- * Clemens Vonrhein, Ph.D. vonrhein AT GlobalPhasing DOT com * Global Phasing Ltd., Sheraton House, Castle Park * Cambridge CB3 0AX, UK www.globalphasing.com *-------------------------------------------------------------- ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
