Hi Kahkashan, Indeed, homodimers formed by engineered disulfides could crystallize readily in novel crystal forms: Banatao et al., "An approach to crystallizing proteins by synthetic symmetrization”, 2006, PNAS, PMID 17050682.
Best wishes, Tomas On Mon, Mar 16, 2020 at 7:58 AM Firdous Tarique <kahkashantari...@gmail.com> wrote: > > Hi > > I have a weird question. Is it possible to incorporate or mutate amino acid > residues at the interface of two interacting surfaces in a multi subunit > protein complexes hoping it to form a disulfide bond making the over all > complex more stable for downstream structural studies ? > > Is there any sort of literature available where creating these types of > modification or kind of protein engineering helped in making a more stable > complex, eventually helping in further structural studies by x-ray or cryoem ? > > Best > > kahkashan > > ________________________________ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
