Dear ccp4 community, I am in my last 3 months of my PhD, working at the Structural Biology Unit at IBMB-CSIC in Barcelona. As my experimental phasing trials using various heavy metals failed so far, we ordered two months ago Expi 293 (Met-) expression medium for selenomethionine labeling (see the reference: https://www.thermofisher.com/order/catalog/product/A4096701#/A4096701. But we got now the notice that it will not be delivered until end of April.
I had been working so hard on this project, and this medium is essential to finished with the experimental part of my thesis. Thus, I was wondering if someone in the community has a bottle (1L) of this expression medium without methionine that I could borrow, and which I would return immediately once our medium arrives. Also in case you have recommendations or other sources of a defined Se-Met medium for Expi293 cells, please let me know. With kind regards from Barcelona, and hope someone can help, Laura --- Laura del Amo Maestro - Proteolysis Lab SBU-Structural Biology Unit, IBMB CSIC Barcelona, Spain, Europe ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
