Actually we don't declare anything to TSA, Jahaun just carries them in his
carryon and it is fine.  Easy peasy?


On Wed, Feb 19, 2020 at 3:48 PM <
00000c2488af9525-dmarc-requ...@jiscmail.ac.uk> wrote:

>
> ... and don't say you're travelling with heavy water!
>
> Jon Cooper
>
> On 19 Feb 2020 17:12, "Azadmanesh, Jahaun" <jahaun.azadman...@unmc.edu>
> wrote:
>
> Hello,
>
> I have traveled to a neutron beamline ~6 times over the past several years.
>
> I have had awful luck shipping crystals, so I decided to hand-carry and I
> found this best.
>
> I'm not sure what conditions you plan to shoot your crystals.  I mount my
> crystals in a sturdy quartz capillary (from vitrocom), seal the ends with a
> layer of wax, then two coats of nail polish (choose your favorite prettiest
> color!)
>
> I would then wrap the capillary in cotton from a cottonball, then "stuff"
> the capillary-cotton combination in a 15 mL falcon tube for a snug fit. For
> extra assurance, the falcon tubes were placed inside a generous amount of
> bubble-wrap that was then taped inside a small cardboard box.
>
> I have sought anaerobic conditions for several of my samples to acquire a
> desired redox state of my metallo-protein. This was tough, so instead I
> used a high-ish concentration of my redox agent dissolved in my reservoir
> solution "slugs" in the capillary. These would be replaced in intervals
> with fresh slugs prior to the beamtime, and for sure right before the
> beamtime. The redox state held for my ~10 days of beamtime and several
> months after.
>
> For cryo-conditions, I just placed a bunch of my crystals in a capillary
> filled with reservoir solution and sealed as above. I would do my chemical
> manipulations and freezing at the beamline.
>
> Much more details are found in our publication:
> https://www.ncbi.nlm.nih.gov/pubmed/30279321
>
> Hope this helps!
> ------------------------------
> *From:* CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of
> stephen.c...@rc-harwell.ac.uk <stephen.c...@rc-harwell.ac.uk>
> *Sent:* Wednesday, February 19, 2020 4:22 AM
> *To:* CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
> *Subject:* [ccp4bb] Shipping samples for neutron diffraction
>
> Non-UNMC email
>
> Dear CCP4 community,
>
>
> I have an impending trip to a neutron source and was wondering how people
> tend to ship their samples prior to beam time?  Is sending something frozen
> best or is sealed in a capillary more sensible or is there another better
> way?  One caveat is that ideally my sample should remain anaerobic which
> has me leaning towards frozen, but I guess in pcr type tubes sealed in gas
> tight vessels is also a viable option?
>
>
> Any thoughts or suggestions are very much appreciated.
>
>
> Best wishes,
>
>
> Steve
>
>
> Dr Stephen Carr
> Research Complex at Harwell (RCaH)
> Rutherford Appleton Laboratory
> Harwell Oxford
> Didcot
> Oxon OX11 0FA
> United Kingdom
> Email stephen.c...@rc-harwell.ac.uk
> tel 01235 567717
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