Hi Jessica You may try the following (if you haven't already):
1. Try solving it using P222 space group. 2. Re-index using different axes. *Regards* *Adarsh Kumar* *Florida State University College of Medicine* *Tallahassee, FL - 32304* On Mon, Dec 16, 2019 at 2:30 PM Jessica Besaw <jbesaw1...@gmail.com> wrote: > There have been two potential space groups: > > P212121 - Rfree = 36% > P21212 - Rfree = 45% > > Xtriage reports that twinning is unlikely. > > Cheers! > > Jessica > > > > > > On Mon, 16 Dec 2019 at 13:56, Jürgen Bosch <jxb...@case.edu> wrote: > >> What’s your spacegroup ? RWork / RFree? >> >> Twinning by any chance? >> >> Jürgen >> >> __________________________________________ >> Jürgen Bosch, Ph.D. >> Division of Pediatric Pulmonology and Allergy/Immunology >> Case Western Reserve University >> 2109 Adelbert Rd, BRB 835 >> Cleveland, OH 44106 >> Phone: 216.368.7565 >> Fax: 216.368.4223 >> https://www.linkedin.com/in/jubosch/ >> >> CEO & Co-Founder at InterRayBio, LLC >> >> Johns Hopkins University >> Bloomberg School of Public Health >> Department of Biochemistry & Molecular Biology >> >> On Dec 16, 2019, at 1:50 PM, Jessica Besaw <jbesaw1...@gmail.com> wrote: >> >> I am crystallizing this membrane protein in a medium (bicelles) that >> forms lamella like sheets that stack on top of each other. >> >> The layer packing is shown below. Is this structure unreasonable? >> >> <Screen Shot 2019-12-16 at 1.40.02 PM.png> >> >> On Mon, 16 Dec 2019 at 13:38, Reza Khayat <rkha...@ccny.cuny.edu> wrote: >> >>> Hi Jessica, >>> >>> >>> The gap between the two proteins is a bit troubling. Perhaps it's the >>> image, but why would a crystal form if there is no crystal contact between >>> the two proteins? >>> >>> >>> Reza >>> >>> >>> Reza Khayat, PhD >>> Assistant Professor >>> City College of New York >>> Department of Chemistry >>> New York, NY 10031 >>> ------------------------------ >>> *From:* CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Ashish >>> Kumar <mail2ashish...@gmail.com> >>> *Sent:* Monday, December 16, 2019 1:24 PM >>> *To:* CCP4BB@JISCMAIL.AC.UK >>> *Subject:* [EXTERNAL] Re: [ccp4bb] 1 out of 2 proteins in asymmetric >>> unit does not fit density >>> >>> Hi Jessica, >>> >>> It may be possible because of wrong MR solution as well. How were your >>> stats after MR. >>> Also it is correct that it could be possible because of wrong space >>> group. >>> Try changing the Space group and repeat MR. >>> >>> Best Regards >>> Ashish >>> >>> On 16 Dec 2019 22:56, "Jessica Besaw" <jbesaw1...@gmail.com> wrote: >>> >>> Dear community, >>> >>> I am having a lot of trouble solving a protein structure. I think my >>> problem may caused by incorrectly placed proteins in molecular replacement. >>> I have two proteins in my asymmetric unit. It appears that one protein fits >>> perfectly, and the other one has many errors. (See snapshots below). I have >>> tried deleting the parts of the protein (and even the whole protein) to try >>> and rebuild it in COOT, but it was a bit too difficult for me to solve. >>> >>> I would appreciate any and all suggestions for potential strategies >>> moving forward. >>> >>> Other information: >>> (1) 2.4 Angstrom >>> (2) 99% complete >>> (3) "Translational NCS may be present at a level that may complicate >>> refinement" >>> >>> Cheers! >>> >>> Jessica >>> >>> <Screen Shot 2019-12-16 at 11.58.10 AM.png> >>> <Screen Shot 2019-12-16 at 11.58.31 AM.png> >>> >>> >>> >>> >>> >>> >>> >>> ------------------------------ >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >>> <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=MBjWbF0ZDC5tg1IQYg3-zjOPSn7yuF2KfXIxak9l0MA&s=E2hs1sz4cNOm0vRwjsoHzxqEyBnMO-5BfM18hOltqLI&e=> >>> >>> >>> >>> ------------------------------ >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >>> <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=MBjWbF0ZDC5tg1IQYg3-zjOPSn7yuF2KfXIxak9l0MA&s=E2hs1sz4cNOm0vRwjsoHzxqEyBnMO-5BfM18hOltqLI&e=> >>> >>> ------------------------------ >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >>> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> >> >> > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
