Besides detergents and I do not know what secret ingredients, the
mixture includes *two* proteolytic enzymes.
If I were to design the mixture for needle cleaning, to digest different
types of proteins, I would probably not use a costly enzyme with high
substrate specificity, I would use proteases as found in washing powders
such as savinase and neutrase (metalloenzyme) or any other combination
of two out of six enzymes as offered by Novo Nordisk......
Jeroen
Am 09.09.19 um 00:01 schrieb Jorg Stetefeld:
Hi everyone,
this is Joerg Stetefeld.
I would like to approach the community in a peculiar case of a
specific proteolytic cleavage during crystallization attempts.
Performing several crystallization screens we figured out that ZYMIT
(https://www.ipcol.com/cleaners/zymit-low-foam) is the reason for a
highly specific, but undesireable cleavage of protein components in
our setups. Most remarkably, the cleavage site of our crystallisation
target is not described in any protease database, and is highly
inaccessible.
According to a publication by Naschberger /et al/, 2014
(doi:10.1107/S2053230X14026053) the authors very nicely describe the
phenomenon. They also describe a very elegant protease removal
protocol, which works in our hands very well.
At this point, however, I would like to know what is “behind” ZYMIT?
Naschberger /et al/ say “Zymit contains an unspecified‘protease
enzyme’ as a trade secret (http://www.ipcol.com/pdfs/Zymit_msds.pdf)”.
Does anyone has more insight into the secrets of ZYMIT? Our attempts
to contact several vendors here in Canada/ abroad failed and a further
characterisation of its nature would help us to understand this
particular phenomenon of proteolytic cleavage.
Thank you very much in advance- Your advise is appreciated.
js
Jörg Stetefeld
**
<https://stetefeldlab.ca/>https://stetefeldlab.ca/<https://stetefeldlab.ca/>
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*Dr.math. et dis. nat.Jeroen R. Mesters*
Deputy, Lecturer, Program Coordinator /Infection Biology
/
<http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html>Visiting
Professorship (South Bohemian University) in Biophysics
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*University of Lübeck*
Center for Structural and Cell Biology in Medicine
*Institute of Biochemistry*
Tel +49 451 3101 3105 (secretariate 3101)
Fax +49 451 3101 3104
jeroen.mest...@uni-luebeck.de <mailto:jeroen.mest...@uni-luebeck.de>
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