Natesh,
Did you use an experimentally determined dn/dc value?
Is it a glycoprotein, or does it have some other modification?
Default dn/dc values are not appropriate for glycoproteins, and will give rise
to incorrect Mwt values.
Dave
--
Dr David C. Briggs
Senior Laboratory Research Scientist
Signalling and Structural Biology Lab
The Francis Crick Institute
London, UK
==
about.me/david_briggs
________________________________
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Natesh
Ramanathan <nat...@iisertvm.ac.in>
Sent: Tuesday, August 27, 2019 6:57:18 AM
To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] SEC and MALS
Dear Friends,
Can you share your experience with examples of MALS giving lower
molecular weight (Eg. Monomer) and SEC giving higher molecular weight (Eg.
Dimer), for the same protein sample?
If you have/know any published paper, can you please point me to that
reference paper or send me the paper?
Many thanks.
Best regards,
Natesh
--
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Dr. Ramanathan Natesh
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Indian Institute of Science Education and Research Thiruvananthapuram
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