Well - there are lots of ways to proceed. It doesnt really matter for the crystallography theory if the exptl phases are from SAD or SIR. Just harder to handle in some software I think I would start to refine the poor MR model with the xptl phases as restraints. REFMAC will do this. Then see if the maps look better - can you see features not in the model?.. If they do you could use Buccaneer to try to rebuild the existing model using exptl phases.. It might work! Eleanor
On Tue, 23 Jul 2019 at 09:55, Luke Smithers < [email protected]> wrote: > Hi all, > > I have collected native data and data on a bromide derivative of one of my > crystals, but have struggled to get a second derivative. I went through the > SIR pipeline as the anomalous signal from the Br was weak and have > generated some not-so-great phases. I have also attempted MR with the > closest match in the PDB (which is only 22% sequence identity) and got some > more not-so-great phases. I have found plenty of programs that will combine > SAD/MR phases, is there anything that will do SIR/MR? I understand it's > likely I will need more and better data and ideally a second derivative but > thought it was worth putting it out there to see if there is something like > this. > > Luke > > -- > > *Luke Smithers* > > *PhD Candidate* > > Protein Production and Structure Facility, School of Molecular Sciences > > *T *+61 8 6488 3163 > > [image: The University of Western Australia] > <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/uwa-logo/> > > [image: Pursue Impossible] > <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/pursue>[image: > Facebook] > <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/facebook>[image: > Twitter] > <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/twitter>[image: > Youtube] > <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/youtube> > > [image: > http://static.weboffice.uwa.edu.au/visualid/graphics/email/sig2015/campaign.gif] > <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/campaign> > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
