The structure of milk proteins obtained from in vivo-grown crystals from a viviparous cockroach could also serve as an interesting case:
http://dx.doi.org/10.1107/S2052252516008903 <http://dx.doi.org/10.1107/S2052252516008903> best wishes Savvas > On 24 Jun 2019, at 16:03, Holton, James M > <0000270165b9f4cf-dmarc-requ...@jiscmail.ac.uk> wrote: > > A classic case of this is crambin. Residue 25 of 3nir. > > -James Holton > MAD Scientist > > On 6/24/2019 1:00 AM, Guenter Fritz wrote: >> Dear all, >> >> I am refining a multimer and mass spec data of the sample indicate >> that there is a mixture of two variants which differ in one amino acid >> residue. The density that we see is therefore most likely an average >> of both variants. I have created a pdb file with "alternate residues" >> each with 0.5 occupancy at this position to use it in refinement. >> However, the programs detect this as an error in the pdb file. >> >> Has anyone faced such a problem previously? Any suggestions are very >> much appreciated. >> >> Thanks a lot and best regards, Guenter >> >> ######################################################################## >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
