Hi community, I've been trying to use Relion 3.0 beta2's own Motion Correction to process ~4000 movies collected by K3 camera. However, it stopped in the middle after 40 hours run with errors shown below. Does anyone has experience with it?
Thanks in advance for your advice! Yu Qiu rawTIFF/A1new_02533.tif: too few valid local trajectories to fit local motion model. rawTIFF/A1new_02542.tif: too few valid local trajectories to fit local motion model. rawTIFF/A1new_02536.tif: too few valid local trajectories to fit local motion model. TIFFFetchDirectory: Can not read TIFF directory count. TIFFReadDirectory: Failed to read directory at offset 152370858. TIFFFetchDirectory: Can not read TIFF directory count. TIFFReadDirectory: Failed to read directory at offset 152370858. TIFFFetchDirectory: Can not read TIFF directory count. TIFFReadDirectory: Failed to read directory at offset 152370858. TIFFFetchDirectory: Can not read TIFF directory count. TIFFReadDirectory: Failed to read directory at offset 152370858. TIFFFetchDirectory: Can not read TIFF directory count. TIFFFetchDirectory: TIFFFetchDirectory: Can not read TIFF directory count. TIFFReadDirectory: Failed to read directory at offset 152370858TIFFFetchDirectory: Can not read TIFF directory count. Can not read TIFF directory count. TIFFFetchDirectory: Can not read TIFF directory count. TIFFReadDirectory: Failed to read directory at offset 152370858. TIFFFetchDirectory: Can not read TIFF directory count. TIFFReadDirectory: Failed to read directory at offset 152370858. TIFFReadDirectory: TIFFFetchDirectory: Can not read TIFF directory count. TIFFReadDirectory: Failed to read directory at offset 152370858. TIFFFetchDirectory: Can not read TIFF directory count. TIFFReadDirectory: Failed to read directory at offset 152370858. TIFFReadDirectory: Failed to read directory at offset 152370858. Failed to read directory at offset 152370858. . TIFFReadDirectory: Failed to read directory at offset 152370858. rawTIFF/A1new_02534.tif: too few valid local trajectories to fit local motion model. rawTIFF/A1new_00458.tif: too few valid local trajectories to fit local motion model. rawTIFF/A1new_00467.tif: too few valid local trajectories to fit local motion model. TIFFFetchDirectory: Can not read TIFF directory count. TIFFReadDirectory: Failed to read directory at offset 27030974. TIFFFetchDirectory: Can not read TIFF directory count. TIFFReadDirectory: Failed to read directory at offset 27030974. in: /programs/x86_64-linux/relion/3.0_beta_cu8.0/src/motioncorr_runner.cpp, line 1209 === Backtrace === /site/ne/app/x86_64/relion/v3.0/bin/relion_run_motioncorr(_ZN11RelionErrorC1ERKSsS1_l+0x41) [0x41f211] /site/ne/app/x86_64/relion/v3.0/bin/relion_run_motioncorr(_ZN16MotioncorrRunner26executeOwnMotionCorrectionER10Micrograph+0x404a) [0x45710a] /site/ne/app/x86_64/relion/v3.0/bin/relion_run_motioncorr(_ZN16MotioncorrRunner3runEv+0x22c) [0x45762c] /site/ne/app/x86_64/relion/v3.0/bin/relion_run_motioncorr(main+0x3d) [0x41908d] /lib64/libc.so.6(__libc_start_main+0xf5) [0x2aaab9ab6b35] /site/ne/app/x86_64/relion/v3.0/bin/relion_run_motioncorr() [0x419691] ================== ERROR: Too few frames (< 3) after grouping. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
