Hi Mohinder, Have you tried SEC columns like Sephacryl S-400 or CL-4B?
They have a higher fractionation range than Superose 6 HTH, Dave ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Mohinder Pal <mohinder.o....@gmail.com> Sent: 17 April 2019 16:10 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] analytical gel filtration columns Dear all, I would like to gel filter a multi protein complex (1.4MDa) as the final purification step. I have tried tandem Superose 6 columns and this complex elutes close to void volume as it is a very elongated molecule. I was wondering if someone could suggest different analytical columns for better resolution as I plan to add more components to this existing complex. Best wishes, Mohinder Pal ---------------------------------------------- "Whatever you’re meant to do, do it now. The conditions are always impossible.” Doris Lessing ---------------------------------------------- ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwebadmin%3FSUBED1%3DCCP4BB%26A%3D1&data=02%7C01%7C%7Cbe1c786feace45cfb96708d6c346edc1%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C0%7C636911106740934606&sdata=5ZQqZ9SR4CybAhVO7oRYkFm0sq6xIAV2r5q4ZFxpXno%3D&reserved=0> The Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a company registered in England and Wales no. 06885462, with its registered office at 1 Midland Road London NW1 1AT ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
