Sorry, I meant to write Why are the values for R(free) in parentheses LOWER than R(factor) in parentheses?
Diana ************************************************** Diana R. Tomchick Professor Departments of Biophysics and Biochemistry UT Southwestern Medical Center 5323 Harry Hines Blvd. Rm. ND10.214A Dallas, TX 75390-8816 diana.tomch...@utsouthwestern.edu<mailto:diana.tomch...@utsouthwestern.edu> (214) 645-6383 (phone) (214) 645-6353 (fax) On Apr 16, 2019, at 1:21 PM, Diana Tomchick <diana.tomch...@utsouthwestern.edu<mailto:diana.tomch...@utsouthwestern.edu>> wrote: Why are the the values for R(free) in parentheses higher than R(factor) in parentheses? I would not expect that to happen, if the same resolution limits for the last shell are used for both values. And I echo the previous commentator, please provide the resolution limits for the values in parentheses. What exactly is R(factor) in this case? The R(work), or the [R(work) + R(free)]? Please define. Diana ************************************************** Diana R. Tomchick Professor Departments of Biophysics and Biochemistry UT Southwestern Medical Center 5323 Harry Hines Blvd. Rm. ND10.214A Dallas, TX 75390-8816 diana.tomch...@utsouthwestern.edu<mailto:diana.tomch...@utsouthwestern.edu> (214) 645-6383 (phone) (214) 645-6353 (fax) On Apr 16, 2019, at 11:57 AM, Jan van Agthoven <janc...@gmail.com<mailto:janc...@gmail.com>> wrote: Hi everyone, I’m trying to publish two structures at 3.1Å resolution with the following refinement statistics: Resolution range (Å) 49.2-3.1 49.3-3.1 Rfactor (%) 24.0 (32.4) 23.4 (32.0) Rfree (%) 26.6 (29.2) 26.3 (31.6) Data collection Completeness 100 (100) 100 (100) Redundancy 6.9 (7.0) 6.2 (6.3) Molecules in asymmetric unit 1 1 Average I/σ 14.1 (1.7) 15.3 (2.0) Rmerge (%) 14.9 (100) 12.7 (100) Rmeas (%) 16.2 (100) 13.9 (100) Rsym (%) 6.2 (68.6) 5.5 (57.1) Wilson B-factor 65.6 62.7 I’ve been told that the Rfree factor in the last shell are too high. Does anyone know how I can improve these Rfree factors other then cutting the resolution, which already is rather low? ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ________________________________ UT Southwestern Medical Center The future of medicine, today. ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
