Agreed. "PG4" - so that you don't have to go searching for it.
On 02/04/19 00:22, Peat, Tom (Manufacturing, Parkville) wrote: > > Hello Zhen, > > > > PEG comes as a mixture and the weight given on the bottle is just the > average molecular weight, so you don’t need any ‘cleavage’ to have > smaller molecular weight PEGs present in your crystallisation cocktail. > > Try putting a small PEG in, it looks like it might fit. > > Cheers, tom > > > > *From:*CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf > Of *Zhen Luo > *Sent:* Tuesday, 2 April 2019 9:59 AM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] unidentified crescent-shaped electron density > > > > Dear all, > > > > Could you please shed some light on what this crescent-shaped density > around the lysine side chain might belong to? I now have two unrelated > protein structures where this kind of density can be found surrounding > a lysine side chain. > > > > > > One protein was crystallised in 0.1 M CaCl_2 and 20% PEG 3350; the > other in 10% PEG 20000, 20% PEG MME 550, 0.03 M CaCl_2 /MgCl_2 , 0.1 M > MES/imidazole. Protein buffers contained 0.025 M HEPES and 0.15 M > NaCl. None of these fitted in well. Could it be cleaved PEG? > > > > Any suggestion would be greatly appreciated. Thanks in advance! > > > > Best regards, > > Zhen Luo > > > > School of Chemistry and Molecular Biosciences > > The University of Queensland, Australia > > > > > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
