Re: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Refinement

[Wim Burmeister]

Hello, I rather think the model has a problem. Are there large domains which are invisible in the structure ? Is it possible that the data are twinned ? Did you use TLS refinement to account for anisotropy ? Just cutting the resolution will probably not help, unless there is a serious problem in data quality. Best Wim On 25/03/2019 13:31, Sharan Karade wrote: As I understand helical assembly, the data some time is anisotropic (any one or two a*,b* or c* has more diffraction than other), which may be responsible for your high Rfactors. You can crop the data to the resolution using softwares. Regards Sharan On Mon, Mar 25, 2019, 8:17 AM Andreas Förster <docandr...@gmail.com> wrote: Yeah, good stuff: http://www.auspex.de/ All best. Andreas On Mon, Mar 25, 2019 at 10:45 AM CCP4BB <0000193323b1e616-dmarc-requ...@jiscmail.ac.uk> wrote: Even if you can't see them, it may be worthwhile investigating with Auspex (Parkhurst, Thorn, Winter & Waterman - sorry, I can't remember the reference off-hand). There's an easy to use webserver somewhere that runs it... Harry -- Dr Harry Powell On 25 Mar 2019, at 09:03, herman.schreu...@sanofi.com wrote: Dear ???, Do you have any ice rings (even hardly visible ones) in your diffraction data? Best, Herman   Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von StrBio Gesendet: Sonntag, 24. März 2019 05:17 An: CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] [ccp4bb] Refinement   ALL.   I have data at 2.4 A in P21 sp gr, helical protein. Refined to Rwork 29 Rfree 34 with nice density map and all nice statistics oither Rfactor (by Phenix). Refmac quit same. Should I deposit it or look better data? Any suggestion?       To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 -- Wim Burmeister Professeur Institut de Biologie Structurale (IBS) CIBB 71 avenue des Martyrs / CS 20192 38044 Grenoble Cedex 9, FRANCE E-mail: wim.burmeis...@ibs.fr Tel:    +33 (0) 457 42 87 41       Fax: +33 (0) 476 20 94 00 website                                    map Changement climatique : «Les autres combats n’ont aucun sens si celui-là est perdu» (Aurélien Barrau)            To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1