10% of which beam? -James Holton MAD Scientist
On Feb 26, 2019, at 1:38 PM, Maria Håkansson <maria.hakans...@saromics.com<mailto:maria.hakans...@saromics.com>> wrote: Dear all, We have experienced nitril groups not present in the electron density after data collection on compounds bound to different proteins. In one of the projects the compound was tested by MS to check that the nitril is present in solution before data collection (crystals used to for MS analysis). The nitril should be present since the molecular weight of the compound is the same. Also the crystals have been exposed using 10% of the beam (instead of 100%) to try to make the effect of radiation damage as small as possible. Still the nitril is not present. In both cases the nitril atoms have been modeled with high temperature factors but the electron density is missing, see parts of the compound in the image below contoured at 1 sigma level and in a 1.1 Å electron density map. Has anyone else experienced this? Best regards, Maria Maria Håkansson, PhD, Crystallization <Screenshot 2019-02-26 at 13.34.17.png>Facility Manager Principal Scientist SARomics Biostructures AB Medicon Village SE-223 81 Lund, Sweden Mobile: +46 (0)76 8585706 Web: www.saromics.com<http://www.saromics.com> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
