We used to use dichlorodimethylsilane in toluene to siliconize both
coverslips and the special glass capillaries for crystal mounting.
I don't have the protocol we used anymore, but the one listed on
protocolpedia sounds familiar.
https://www.protocolpedia.com/blog/2017/05/11/siliconized-coverslips-2/
I recall we often baked the items afterwards.
We used to use staining racks to hold the coverslips and a staining
vessel to hold the solution (in a hood). The racks looked like
https://www.thomassci.com/Equipment/Histology/_/THOMAS-COVER-GLASS-STAINING-OUTFITS?q=Cover%20Glass%20Rack
it looks like sigma also has a poly-propylene rack that is much
less expensive,
https://www.sigmaaldrich.com/catalog/product/sigma/z688568?lang=en®ion=US&cm_sp=Insite-_-recent_fixed-_-recent5-5
but they may be incompatible with toluene.
Regards,
Mitch
Quoting Zhijie Li <zhijie...@utoronto.ca>:
Hi,
I believe that one can put a 50-100uL drop of fresh SigmaCote (in a
tube cap) with the glass pieces (surface well exposed), sealed in a
dedicated (because the container will be coated too) container
(air-tight lunch boxes). After a while the SigmaCote vapor should
react with the glass and bring polysiloxane groups to the glass
surface. I have done this with microscope slips. To make a few
hundred .22 mm cover slips I think the major challenge is to make a
frame for supporting the separated (~1mm apart to allow free
diffusion?) cover slips (3D print?). Dry paper may work too. If some
of the cover slips tend to stick together, a little vacuuming can
help.
SigmaCote is a chlorinated polysiloxane. The Cl-Si group allows it
to react with the hydroxyls on glass surface. Just be aware that the
same group reacts even more readily with water in air, making the
reagent less reactive with glass overtime (but I have used a VERY
old bottle and it worked).
References:
https://en.wikipedia.org/wiki/Chlorosilane
https://pubs.acs.org/doi/pdfplus/10.1021/j100727a046
https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/1/sl2pis.pdf
Zhijie
On 31/01/2019 4:16 a.m.,
herman.schreu...@sanofi.com<mailto:herman.schreu...@sanofi.com> wrote:
A long time ago, before siliconized coverslips became commercially
available, we used to siliconize coverslips ourselves. It is not
really that much work and unsiliconized cover slips should be very
cheap. If you wish, I could try to find back the protocol.
Best,
Herman
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag
von Rajnandani Kashyap
Gesendet: Donnerstag, 31. Januar 2019 09:17
An: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Betreff: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized
glass coverslips for crystallization?
Dear All
I am a PhD student who requires lots of coverslips (!!) for setting
up hanging drop crystallization. The company sells it for a huge
amount. Also there is a wide monetary difference between a normal
siliconized coverslip and a 22mm siliconized circle coverslips. We
tried to search for an alternative companies but couldn't get any
one who sells coverslips with the same dimensions (0.19-0.22mm glass
thickness and 22 mm glass diameter). Is there any alternative
company (distribution in India) from where we can buy them for a
reasonable price?
Thanks in advance for sparing your valuable time and efforts.
Regards
Rajnandani Kashyap
India
________________________________
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=YlILrL-rg1OZBp9DSEAcOqrUs8BY0vFG_gPlaAB3qNk&s=t6ZqJh8ledXY32ENHFaeBd9vgbGKNUke1ROmyJzdM6k&e=>
________________________________
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
