Hi All, Sorry to bring this old topic up again. I planned to run tricine gels but I found a possible error in table 2 (4% stacking gel formula) in Hermann Schägger protocol (Nature Protocols volume 1, pages 16–22 (2006), the author wrote 3ml 3X gel buffer in a total of 12 ml solution, it should be 4ml 3X gel buffer in a total of 12 ml solution right?
I tried to contact the author but unsuccessful, I do not know if anyone in this forum has noticed if this is an error or not. Xiao On Thu, Jan 19, 2017 at 8:00 AM Didier Spittler <spittlerdid...@gmail.com> wrote: > Yes Tris-Tricine gel ! > > Try to obtain this article from nature protocol. > > Best, > > Didier > > > 2017-01-19 14:47 GMT+01:00 zeyaul islam <zeya1...@gmail.com>: > >> Try Tricine gel. It is particularly suited for low molecular wt proteins >> and it will give you very good resolution. Even you can run it overnight at >> 30 V (16-18 hours). >> >> On Thu, Jan 19, 2017 at 9:33 AM, Walt <ofe...@gmail.com> wrote: >> >>> Hi, >>> >>> I have a small protein (~9 kDa) with acidic pI (~4). >>> When I run 18% native-PAGE, it appears my protein is in the dye front. >>> How can I fix this problem? Changing the pH of separating gel >>> might help? How about gradient native-PAGE? Thank you! >>> >>> Walt >>> >> >> > > > -- > Didier Spittler, PhD > Phone number : +33658576481 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
