The Fischer Lab is recruiting an Associate Scientist for a project that exploits the protein conformational landscape for ligand discovery in pediatric cancer.
We are looking for applicants with a strong training in molecular biology, protein biochemistry, and structural biology and a keen interest or ideally experience in ligand discovery. You will be part of a young, interdisciplinary team that uses structural biology (multi-temperature and high-throughput crystallography) to characterize disease-relevant protein states, computational methods (MD, docking, custom software) to analyze and exploit them, and experiments (biophysical, biochemical, cell-based assays) to test the impact of small molecules on biology and disease. These approaches have relevance for allosteric regulation of proteins and protein-protein interactions. The successful candidate has these attributes: 1) Must be able to conduct independent research in molecular biology and protein biochemistry, including cloning and protein purification, that enable structural studies. 2) Demonstrated experience in protein crystallography. 3) Familiarity with large-scale/ high-throughput approaches, automation, liquid handling, biophysical assays or computational approaches is a plus. 4) Proficiency in e.g. python or shell scripting will facilitate scaling up data analysis. 5) Must be eager to learn and implement new techniques from those relevant areas. Besides working on your own projects, you will form a liaison for other lab members and support projects within the lab while maintaining efficient laboratory operations. Our group is housed in the Department of Chemical Biology and Therapeutics ( https://www.stjude.org/research/departments-divisions/chemical-biology-therapeutics.html), and Structural Biology ( https://www.stjude.org/research/departments-divisions/structural-biology.html) at St. Jude Children’s Research Hospital. With ongoing investments into exceptional structural and chemical biology facilities, this is an exciting time to join our lab to do cutting-edge research whilst living in an affordable city. Stable funding for this post is available. *Minimum Experience:* Bachelor’s plus 15 years post-degree relevant experience, with at least 7 years at the Research Lab Specialist level OR Master’s plus 14 years post-degree relevant experience, with at least 7 years at the Research Lab Specialist level OR PhD plus 9 years post-degree relevant experience (could include post-doc and/or technical staff experience) Please direct your questions and application package including a cover letter detailing your interest in the lab, current CV, and 3 letters of reference to: Dr. Marcus Fischer (marcus.fisc...@stjude.org). Relevant papers include: • Fischer et al. (2015). One crystal, two temperatures: cryocooling penalties alter ligand binding to transient protein sites. 1560-64. PMID 26032594 • Merski et al. (2015). Homologous ligands accommodated by discrete conformations of a buried cavity. PNAS 112, 5039-44. PMID 25847998 • Fischer et al. (2014). Incorporation of protein flexibility & conformational energy penalties in docking screens to improve ligand discovery. Nature Chemistry 6, 575-83. PMID 24950326 Link to ad: https://careers-stjude.icims.com/jobs/3740/associate-scientist/job -- Marcus Fischer, PhD Assistant Member Chemical Biology & Therapeutics and Structural Biology https://www.stjude.org/fischer ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
