I would say so yes, but Garib is the expert on this - I'm only repeating what he told me!
Cheers -- Ian On 15 March 2018 at 14:41, Eleanor Dodson <eleanor.dod...@york.ac.uk> wrote: > So if I calculate "structure factors" from a map do I just use the map as > is with the negative values included ? > > Eleanor > > On 15 March 2018 at 14:37, Ian Tickle <ianj...@gmail.com> wrote: > >> >> Hi Eleanor >> >> Electron scattering factors can be negative for negative ions, >> particularly at low d*. For a low-resolution map it means that the >> electric potential is the opposite sign to what you expected. It's why you >> often don't see ASP & GLU side-chains in low resolution EM maps. >> >> Cheers >> >> -- Ian >> >> On 15 March 2018 at 14:17, Eleanor Dodson <0000176a9d5ebad7-dmarc-reques >> t...@jiscmail.ac.uk> wrote: >> >>> I am pig-ignorant about these ,, but this example has negative values as >>> well as positive.. >>> >>> What does this mean? I thought a well phased map would be pretty well >>> all positive.. >>> >>> Eleanor >>> >> >> >
