Dear Peng,
to me your problem sound a bit strange, except if it's a palindromic
sequence. I don't understand how you can have one part of the DNA in one
asymmetric unit and one in another one. My question are : maybe you
considering a NCS as a true symmetry and underestimate the unit-cell
dimensions? Or you DNA has been degraded by DNase and the current size
is not 20pb, in this case you are not supposed to create an artificial
connection. Is the resolution good enough to be certain of the sequence ?
Sorry, I don't provide any answer, but I am curious and try to
understand what is going on.
Hope this finally help.
Nicolas
Nicolas Foos
PhD
Structural Biology Group
European Synchrotron Radiation Facility (E.S.R.F)
71, avenue des Martyrs
CS 40220
38043 GRENOBLE Cedex 9
+33 (0)6 76 88 14 87
+33 (0)4 76 88 45 19
On 29/01/2018 20:28, Peng wrote:
Hello, everyone，
Recently, we solve a protein-DNA complex. 20bp-DNA was used for
crystallization, but only 6bp was found in one symmetric unit.
My question is：
How to define the DNA bond between P and O3’ from two different
symmetric units during my refinement?
Peng
