Hello,
Welcome to the club of unexpected results!
You don't provide a lot of background, but based on what you wrote you can:
1. Do a BLAST search using a known part of your sequence to find whether
this sequence has been deposited.
2. Assign the different residues based on the chemical environment and
electron density and refine the structure.
I'm sure you can submit the refined structure to the PDB even from an
unknown protein.
Regards,
Pedro Matias
Às 12:08 de 14/12/2017, Jiyong Su escreveu:
> Dear CCP4bb,
>
> In 2014, I collected a high quality data set from a crystal. But I
> could not solve the structure of that crystal because this protein is
> a contaminate.
> Recently, I used StruBE's Contaminer and fortunately got the solution.
> Thanks ContaMiner!!! This protein is a contaminate protein.
>
> However, I found this protein is an unknown protein (about 180
> residues) whose amino acid sequence is not totally same as E.coli.
> There are about 20 point mutation sites comparing to the E.coli
> protein. This means this protein may be from an unknown bacteria.
>
> The space group of this crystal is new. There is also a new ligand in
> this protein.
>
> My question is how could I found the primary structure of this protein
> and how to deposit this protein in PDB.
>
> Best regards,
>
> Jiyong
>
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