Hi, Tristan Croll, Thanks for your suggestion.I will consider to rebuild these first.
Yang > 在 2017年10月10日，下午9:18，Tristan Croll <ti...@cam.ac.uk> 写道： > > Relying on refinement to fix cis peptide bonds for you is unlikely to end > well. It looks to me like you really need to spend some time investigating > and manually rebuilding these first. > > On 2017-10-10 13:52, 师扬 wrote: >> Dear all, >> I am refining a model based on a 4.3A EM density map,and there are >> some cis-peptides in the beginning model. >> By using phenix.real_space_refine with a very low cis-peptide >> threshold (0), all the cis-peptide become to the twisted. >> The start Omega angle: >> cis-proline: 31.63 % >> twisted proline: 0.00 % >> cis-general: 11.11 % >> twisted-general: 0.05 % >> The final Omega angle: >> cis-proline: 0.00 % >> twisted proline: 27.55 % >> cis-general: 0.00 % >> twisted-general: 6.04 % >> My questions are: >> 1) What is the twisted peptide? >> 2) Is the amount acceptable at the current resolution? >> 2) How to refine it? >> Thanks in advance! >> Yang Shi >> 【网易自营|30天无忧退货】仅售同款价1/4！MUJI制造商“2017秋冬舒适家居拖鞋系列”限时仅34.9元>> >> [1] >> Links: >> ------ >> [1] http://you.163.com/item/detail?id=1165011&from=web_gg_mail_jiaobiao_9
