Our RPA14/32 crystals from 2007 included full length protein for both subunits (RPA14 and RPA32), but only the central OB fold of RPA32 could be modelled. The crystals included RPA32(1-270) but only 42-176 could be modelled. I remember being very frustrated by not being able to visualize the wHLH domain at the CT or RPA32. There is disordered density for the rest due to the flexible linkers. All of RPA14 could be modelled. This happened for all 3 crystal forms we solved... see 2PI2, 2PQA, and 2Z6K
On Mon, Oct 2, 2017 at 9:22 AM, zaigham mahmood khan <mahmood.zaig...@gmail.com> wrote: > Hey Rajesh > > You may try the following link: > > Please enter the protein sequence, and scroll down the "Select standard > database" tab, and choose "pdb_nr". Once you will "submit" the job, you will > most likely get what you want to see! > > You may pick any pdb ID from the result section, and from pdb.org, you may > find the residues of that protein that were observed in the electron density > map as well as whole expression casstte that was attempted to crystallize > for that particular pdb entry. > > Best wishes > > -Z > > > Zaigham Mahmood Khan, PhD > > Icahn School of Medicine at Mount Sinai > Department of Oncological Sciences > 1470 Madison Avenue > New York > > On Wed, Sep 27, 2017 at 6:47 PM, Rajesh > <00001642be9504b8-dmarc-requ...@jiscmail.ac.uk> wrote: >> >> Dear BB, >> >> Sorry for the off topic. >> >> Does anyone know how to search the PDB for the entries that have the >> density only for part of the protein molecule rather than for the entire >> length of the protein attempted to crystallize? >> >> >> >> Thanks, >> Rajesh.. > >
