Hi all, a group in the department here has a sample with high amount of maltodextrins (~40 mg/ml) that they would like to load on a superdex column. Anyone has advices on whether this is doable / not recommendable / absolutely to avoid? So far, I have just found that people are separating dextrans on superdex columns, but their elution buffer is supplemented with 5% ethanol. Is that added to avoid specific binding of the sugars to the column matrix? Is that necessary or can it be excluded? Thanks a lot in advance for any hint, ciao, Sebastiano
-- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990 web http://is.gd/IEOXtalUnit
