Dear Nishant, You can use anything you want as a molecular replacement search model including NMR models/ensembles or homology models. Whether or not it will be successful is another matter!
For borderline cases, some have reported success using Phaser in combination with the Rosetta modelling suite. E.g. https://www.phenix-online.org/documentation/reference/mr_rosetta.html HTH, Dave -- Dr David C Briggs Hohenester Lab Department of Life Sciences Imperial College London UK http://about.me/david_briggs From: Nishant Varshney Sent: Tuesday, 29 August, 11:51 Subject: [ccp4bb] NMR or Homology Model as a MR model To: ccp4bb@jiscmail.ac.uk Dear Crystallographers, I am working to solve an human protein structure which has a domain sequence identity of 24% with domain of another protein. As Phaser as well as Molrep failed to give any definite solution (TFZ=3.7 from MR), I want to ask, if solution structure of another protein having domain sequence similarity of 25% or a homology model can be used as a template for MR? Many thanks and Regards Nishant -- Dr. Nishant Kumar Varshney, Research Associate, C/O Dr. Sameena Khan, Drug Discovery Research Center, Translational Health Science and Technology Institute (THSTI) NCR Biotech Science Cluster, 3rd Milestone, Faridabad – Gurgaon Expressway, Faridabad – 121001 (HARYANA), India Ph: +91- 0129-2876477<tel:+91%20129%20287%206477> Mob: 8390564690
